The Protective Effect of Melatonin on Sperm Parameters, Epididymis and Seminal Vesicle Morphology in Adult Mouse Treated with Busulfan

Authors

  • FaghaniLangroudi, Masoumeh
  • Mohammad Ghasemi, Fahimeh
  • Falah Karkan , Morteza
Abstract:

Purpose: The aim of this study was to investigate the protective effect of melatonin on sperm parameters, epididymis and seminal vesicle morphology in adult mouse under chemotherapy. Materials and Methods: Male adult NMRI mice were divided into four groups. The control group received a single dose of DMSO, Group 2 received a single dose of busulfan 20mg/kg. Group 3 was administered melatonin 10mg/kg for 5 days. Group 4 received a 5 days course of melatonin 10 mg/kg following an initial dose of busulfan 20mg/kg. Animals were sacrificed 35 days after treatment and evaluations were made by determining of sperm count and sperm quality, histological study of epididymis, seminal vesicle and measuring of plasma testosterone level. Statistical analyses were performed using ANOVA and Tuckey test. Results: Busulfan significantly reduced sperm count, sperm motility and normal morphology and testosterone level in comparison with that of control group (P<0.01). However, combined treatment increased mentioned parameters in compare with those of chemotherapy treated group (P<0.01). In histological evaluations busulfan resulted in vacuoles in epithelial thickness of epididymis and reduced epithelial cell height in comparison with that of control group (P<0.001). Busulfan reduced semen fluid and epithelial folds and epithelial cell height in seminal vesicle in comparison with those of control group (P<0.001). However, combined treatment, resulted in recovery and normalization of the epididymis and seminal vesicle.Conclusion: Melatonin has protective effect on epididymal sperm parameters, seminal vesicle and epididymis morphology in mouse under treatment with chemotherapy. Although the mechanism is not clear, it acts probably by decreasing oxidative stresses.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

The Protective Effect of Quercetin on Sperm Parameters and Serum Biochemical Factors in Adult Mice Treated with Dexamethasone

Background and Objectives: Dexamethasone is a synthetic glucocorticoid, which causes oxidative stress in the testicular tissue. This study aimed to investigate the protective effect of quercetin as a powerful antioxidant on sperm parameters and serum biochemical factors in mature adult mice following the treatment with dexamethasone.   Methods: In this experimental study, 24 mature adult male ...

full text

Protective Effect of Spirulina and Phycocyanin on Testicular Tissue and Sperm Parameters in Adult Rats Treated with Hydrogen Peroxide

 Background and purpose: About 50% of infertility in couples is related to male factors and oxidative stress is one of the main causes of infertility in men. Hydrogen peroxide has an adverse effect on male reproductive system due to its free radicals. Spirulina and phycocyanin are known to have antioxidant properties and can modify stressors. Materials and methods: In this experimental study, ...

full text

Preventive and therapeutic role of garlic on the epididymis and seminal vesicle of male diabetic rats

Introduction: Diabetes has adverse effects on reproductive functions in human. Clinical as well as experimental studies revealed impairment of spermatogenesis, reduced volume of seminal fluid and atrophic changes in epididymis. In the present study, we aimed to investigate the preventive and therapeutic effect of garlic juice on epididymal and vesicle seminal damage in male rats with stre...

full text

Protective effect of royal jelly on the sperm parameters and testosterone level and lipid peroxidation in adult mice treated with oxymetholone

Objectives: The aim of the present study was to evaluate protective effect of royal jelly on sperm parameters, testosterone level, and malondialdehyde (MDA) production in mice. Materials and Methods: Thirty-two adult male NMRI mice weighing 30±2 g were used. All the animals were divided into 4 groups. Control group: received saline 0.1 ml/mouse/day orally for 30 days. Royal Jelly group (RJ): re...

full text

the effect of task cognitive complexity and time limit on the performance of the learners with different proficiency levels in second language writing skill

this study investigated the effects of manipulating the cognitive complexity of tasks along +/- few elements and the time limits on l2 learners writing performance. to conduct the study, 60 iranian efl learners with two levels of proficiency, low and high intermediate, were selected and assigned to three groups based on the time devoted to task completion. the participants performed both a simp...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 8  issue 30

pages  0- 0

publication date 2010-05

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023